- Research article
- Open Access
Relative contribution of muscle strength, lean mass, and lower extremity motor function in explaining between-person variance in mobility in older adults
BMC Geriatrics volume 20, Article number: 255 (2020)
Approximately 35% of individuals > 70 years have mobility limitations. Historically, it was posited lean mass and muscle strength were major contributors to mobility limitations, but recent findings indicate lean mass and muscle strength only moderately explain mobility limitations. One likely reason is that lean mass and muscle strength do not necessarily incorporate measures globally reflective of motor function (defined as the ability to learn, or to demonstrate, the skillful and efficient assumption, maintenance, modification, and control of voluntary postures and movement patterns). In this study we determined the relative contribution of lean mass, muscle strength, and the four square step test, as an index of lower extremity motor function, in explaining between-participant variance in mobility tasks.
In community-dwelling older adults (N = 89; 67% women; mean 74.9 ± 6.7 years), we quantified grip and leg extension strength, total and regional lean mass, and time to complete the four square step test. Mobility was assessed via 6-min walk gait speed, stair climb power, 5x-chair rise time, and time to complete a complex functional task. Multifactorial linear regression modeling was used to determine the relative contribution (via semi-partial r2) for indices of lean mass, indices of muscle strength, and the four square step test.
When aggregated by sex, the four square step test explained 17–34% of the variance for all mobility tasks (p < 0.01). Muscle strength explained ~ 12% and ~ 7% of the variance in 6-min walk gait speed and 5x-chair rise time, respectively (p < 0.02). Lean mass explained 32% and ~ 4% of the variance in stair climb power and complex functional task time, respectively (p < 0.02). When disaggregated by sex, lean mass was a stronger predictor of mobility in men.
The four square step test is uniquely associated with multiple measures of mobility in older adults, suggesting lower extremity motor function is an important factor for mobility performance.
Mobility limitations, classically characterized by “slowness” when walking and/or when performing physical tasks [1,2,3], are highly associated with fall risk, disability, increased dependency, hospitalization, and mortality [1, 4,5,6,7], in addition to carrying an annual health care cost of ~ 42 billion in the U.S. . Slow gait speed in older adults, in particular, is a strong predictor of mortality . The percentage of older adults affected by mobility limitations is large, as ~ 35% of individuals > 70 years and more than 50% of individuals 85+ years currently have mobility limitation [2, 9, 10].
Previous research in older adults has focused largely on musculoskeletal mechanisms and processes underlying mobility limitations. This focus is likely due to the premise that sarcopenia, the age-related loss of lean mass , results in reduced muscle strength, which is a major contributor to mobility limitations in older adults . However, recent findings from the Sarcopenia Definitions and Outcomes Consortium indicates the “sarcopenia variables” of lean mass and muscle strength only moderately explain limitations in mobility . We postulate that lean mass and muscle strength do not fully discriminate, or predict, mobility limitations because they do not capture “motor function”. Motor function is the ability to learn, or to demonstrate, the skillful and efficient assumption, maintenance, modification, and control of voluntary postures and movement patterns . We assert that motor function is an important contributor to age-related mobility limitations above and beyond the associations found with lean mass and muscle strength.
Accordingly, in the present study we sought to determine the relative contribution of muscle strength, lean mass, and an index of lower extremity motor function (performance time on the four square step test, which is discussed more below), in explaining between-participant variance in measures of mobility in older adults. To this end, we quantified various aspects of mobility (e.g., gait speed, chair rise time, stair climb power, etc.) using objectively measured, laboratory-based assessments. We chose to use the time to complete the four square step test, a multidirectional stepping test, as an index of lower extremity motor function because this test heavily challenges motor planning and initiation, as well as motor sequencing and recall, and incorporates musculoskeletal, and likely, peripheral sensory factors (i.e., strength and joint range of motion of the hip and ankle, as well as sensation/proprioception) that standard knee extensor strength and mass tests do not evaluate [15,16,17,18]. Our a priori hypothesis was the four square step test would be an independent predictor of mobility performance in community dwelling older adults. Overall, our results were consistent with this hypothesis and underscore the role of lower extremity motor function as a critical determinant of mobility in older adults.
General overview of the study and participants
Eighty-nine community-dwelling older adults participated in this study (Total: N = 89, 63–92 years, mean age 74.9 ± 6.7 years; Males: n = 29, 65–90 years, mean age 75.5 ± 6.3 years; Females: n = 60, 63–92 years, mean age 74.5 ± 6.9 years [see Table 1 for complete descriptive statistics]). To be considered for the study, participants had to be ≥60 years of age and have a body mass index (BMI) between 18 and 40 kg/m2. Study participants had to be living independently, and free of major musculoskeletal, neurological, cardiac, pulmonary, renal, psychiatric, and cognitive disease or disorders (see Supplementary Table 1 for a complete list of inclusion and exclusion criteria). All participants had to be willing to undergo a Dual-Energy X-ray Absorptiometry (DEXA) scan. Participants were instructed to abstain from drinking caffeinated beverages for > 4 h prior and alcohol for > 24 h prior to the testing session. The Ohio University Institutional Review Board approved this study, and all study participants had to provide written informed consent in accordance with the Declaration of Helsinki for their participation. Furthermore, this study adheres to the CONSORT guidelines. Data for this report was derived from part of a larger study/dataset (UNCODE Study; NCT02505529).
With respect to data pertinent to this report, we quantified the following outcomes: total and regional lean mass via DEXA, grip strength, isometric and isokinetic (60°/sec) leg extension strength, and time to complete the four square step test. We also quantified various aspects of mobility using objectively measured, laboratory-based assessments. Here, participants completed two locomotor (6-min walk gait speed and stair climb power) and two non-locomotor (5x chair rise time and time to complete a complex functional task) laboratory-based tests. To permit characterization of our study participants, we also quantified short physical performance battery test score [19, 20], moderate-vigorous intensity physical activity via accelerometry , neuropsychological status via the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS ;) and comorbidities via the Charlson Comorbidity Index . Below we describe the methodological details related to our primary variables of interest.
Lean tissue mass
DEXA scans (Hologic Discovery QDR model Series, Waltham, MA, USA) were performed to assess lean mass as per our prior description . A whole-body scan was performed, and whole-body, appendicular, and lower extremity lean tissue mass was determined using the system’s software package (Hologic APEX, Version 4.0.2). Participants were advised to report to the laboratory in a hydrated state and were given scrubs to wear during the scan. Care was taken to follow The International Society for Clinical Densitometry guidelines for positioning during the scan .
Isometric and isokinetic leg extension maximal voluntary contraction (MVC) strength measures were recorded utilizing a Biodex System 4 Dynamometer (Biodex Medical Systems Inc., Shirley, NY). For quantification of isometric MVC strength, participants were seated with their non-dominant leg at 90° flexion and the knee axis of rotation in alignment with the rotational axis of the Biodex torque motor. A lap belt was applied to prevent movement at the hip, and the participant’s non-dominant lower extremity was affixed to a lower extremity lever arm, which was attached to the Biodex torque motor. The torque signal was scaled to maximize its resolution (208.7 mV/Nm; Biodex Researchers Tool Kit Software) and sampled at 625 HZ (MP150 Biopac Systems). Participants received visual feedback of forces on a monitor located 1-m in front of them. Provided with strong verbal encouragement, participants performed three isometric MVCs with 1 min of rest between each effort, and the peak value of these three trials was utilized as the isometric MVC for the analysis.
Isokinetic MVC strength was conducted on the same leg, using the same general set-up, but isokinetic concentric torque was measured at 60°/sec. Six isokinetic trials were performed with 30 s rest between bouts. Isokinetic MVC strength was calculated as the mean of the highest three values of maximal isokinetic torque produced between 90° and 30° of leg flexion. This isokinetic MVC strength testing protocol corresponds to that used in the Health ABC study, which established cut-points for future development of severe mobility .
Grip strength was assessed in a manner similar to that described by Villanfañe (2015)  using a portable JAMAR® Hydraulic Hand Dynamometer; (Model 5030 J1; Lafayette Instrument Co.; Lafayette, Indiana). In short, after demonstrating its use, the research staff measured the participant’s hand and fingers in the dynamometer to appropriately set up the handle position. The test was performed in the upright seated position with the shoulder of the tested arm adducted to the side, the elbow flexed at 90°, and the forearm and wrist aligned in the neutral position. Next, the dynamometer was placed in the participant’s hand and they were asked to squeeze the dynamometer as hard as possible for 3 s and then let go. Trials were performed with 30 s rest between bouts and measurements were recorded to the nearest 0.5 kg. This process was conducted three times per hand, and the dynamometer was set to the zero kg mark before each measurement. If the relative difference between trials was within 10%, no additional trials were required. If not, additional trials were performed until three trials were within 10%. Once completed, the average of the highest 3 trials were recorded for each hand.
Four square step test
The four square step test was conducted in a manner similar to that described by Dite and Temple (2002) , except we asked participants to step over tape instead of canes. Briefly, participants were required to step in a predetermined sequence over four 76-cm-long pieces of white tape, placed in a cross configuration on the ground over dark colored carpet. Participants were instructed to complete the sequence as fast as possible without touching the pieces of white tape and time to task completion was recorded. The trial was considered a failure and repeated if the sequence was not completed correctly, the participant lost their balance, or if a foot touched the tape line. Trials were only repeated if the participant failed and each participant performed 3 trials with 30 s rest between trials. Test scores were taken from the mean of trials 1–3 and the task was measured to the nearest 0.01 s using a stopwatch.
Six-minute walk distance
Participants were instructed to walk as quick and as far as possible in 6 min (on a 60-m course). Specifically, participants were instructed to walk in a straight line for 30 m, complete a 180° turn to the left around a cone and walk back to the starting line, which was an additional 30 m. Once participants arrived at the starting line, they completed another 180° turn to the left around an additional cone and proceeded to repeat this sequence as many times as possible. Markers were set every 3 m to accurately determine the distance completed in 6 min. Participants were told the remaining time at each turn and verbal encouragement was provided. This task was performed once.
Stair climb power was calculated as: power = force x velocity, where force = body mass in kilograms x acceleration due to gravity, and velocity = cumulative stair height/stair climb time. Prior to the start of this test, participants were weighed to the nearest pound, and were instructed to stand at the bottom of a flight of eight stairs (each stair is 7 in. in height). Next, participants were instructed to climb a flight of stairs as quickly as possible and told not to use the handrail unless they were unable to climb the stairs without it. The time required to complete the test was measured using switch mats (Lafayette Instruments Model 63516A) interfaced with a digital timer (Lafayette Instruments Model 54,060). The task was performed twice, was measured to the nearest 0.01 s, and the results were averaged between trials.
5x chair rise
Participants were asked to sit in a chair with a pan height of ~ 45 cms (height includes ~ 4 cms of padding). Participants were instructed to cross their arms, place their hands on the opposite shoulder, and starting from an upright seated position, they were instructed to stand up and then sit back down five times consecutively keeping both feet on the floor throughout the test. The task was performed once and was measured to the nearest 0.01 s using a stopwatch.
Complex functional task
Participants were asked to sit upright on the floor with their legs extended in front of them with their knees bent to ~ 45°. A laundry basket was positioned in front of their feet. Participants were permitted to place their hands on the ground behind themselves if needed or preferred. Starting from the seated position, participants were instructed to transition from sitting on the floor to standing, then lift the laundry basket that weighed a total of 4.5 kg, walk 1.5 m, and place the basket on a 0.75-m high table. For participants who were unable to complete the task or those who took > 30 s to complete the task, a time value of 30 s was assigned. The task was performed twice, was measured to the nearest 0.01 s using a stopwatch, and the results were averaged between trials. If the participant was unable to perform the test a second time, the first attempt was used to represent their value.
The relative contribution of muscle strength, lean mass, and the four square step test (representing the lower extremity motor function construct) in explaining the between-participant variance in measures of mobility (i.e., six-minute walk gait speed, stair climb power, 5x chair rise time, and time to complete a complex functional task) in older adults was assessed via multifactorial linear regression analyses. Independent predictor variables were muscle strength, lean mass, and the four square step test. Notably, measures of muscle strength and lean mass were expressed in a variety of forms so as to maximize the opportunity for these constructs to contribute to the respective models (see Supplementary Table 2 for a complete list). Furthermore, these three variables did not demonstrate collinearity (i.e., none of the predictor variables had associations ≥0.60). Additionally, the variance inflation factor (VIF) was computed to evaluate multicollinearity for each regression model. Note that VIF values under 2.5 are considered acceptable .
To evaluate the independent contribution of each predictor variable, we calculated the semi-partial r2 value (spr2) as it is interpreted as the variance uniquely attributed to a given predictor variable by factoring out the shared variance from all other variables . We recognize that the relative contribution of the constructs of lean mass and muscle strength could vary depending on how it is expressed (e.g., an absolute value vs. a value relative to body stature), as well as various nuances of what is being measured (e.g., grip strength vs. isometric leg extensor strength vs. isokinetic leg extensor strength; upper thigh lean mass vs. appendicular lean mass, etc.). Thus, in our analyses we expressed lean mass and muscle strength constructs several different ways (see Supplementary Table 2 for a complete list).
The regression models presented herein are those with the “best” model fit characteristics, not necessarily the ones with the “best” independent contribution of the predictor variables. To determine the model of best fit for each dependent variable, we independently evaluated the Schwarz Bayesian Criterion (SBC)  and the Akaike’s Information Criterion (AIC)  for goodness-of-fit for each individual model and not across models. The models with the “best” fit (i.e., closer to 0, or even below 0) are presented in the results. Statistical Package for Social Sciences (SPSS) version 25 (SPSS Inc., Chicago, IL) was used for all statistical analyses, all data was expressed as mean ± SD for descriptive statistics, and all statistical tests were set at the 5% significance level (2-tailed).
If data was missing for a participant, we used an imputation statistical software package from SPSS to run a Markov Chain Monte Carlo algorithm, known as fully conditional specification or chained equations imputation. The Markov Chain Monte Carlo method was used because multivariate normality was acceptable . The algorithm imputes incomplete variables one at a time, using the filled-in variables as a “pooled output” to estimate what the value would have been if the original dataset had no missing values . The imputation software was only used when 10% or less of the population were missing values for a particular variable. Thus, we could only allow missing values for up to eight participants for any given variable. Notably, no variables had missing values > 10% of the study population. In addition, we conducted a sensitivity analysis with and without the missing values and found there no statistical difference.
Post-hoc estimation of statistical power and effect size was determined for the overall model using the smallest R2 value of 0.5 (5x chair rise model), an α = 0.05, a n = 89, and three predictors, which resulted in a post-hoc power of 0.99. For the male-specific model, post-hoc estimation of power was based on the smallest R2 value of 0.29 (CFT model), an α = 0.05, a n = 23, and three predictors, which resulted in a post-hoc power of 0.73. Lastly, for the female-specific model, post-hoc estimation of power was based on the smallest R2 value of 0.42 (stair climb power model), an α = 0.05, a n = 60, and three predictors, which resulted in a post-hoc power of 0.99. All post-hoc power calculations were conducted using G*Power 3.1 .
Overall model results (Table 2)
The results when both men and women were included in the models are displayed in Table 2. The overall R2 values explained by the four square step test, muscle strength, and lean mass, for 6-min walk gait speed, stair climb power, 5x chair rise time, and the time to complete the complex function task, were 0.66, 0.66, 0.50, and 0.53, respectively (p < 0.01). The four square step test uniquely explained 17–34% of the between subject variance for all the mobility tasks (i.e., spr2 values = 0.17–0.34; p < 0.01) (Fig. 1). Muscle strength uniquely explained ~ 12% and ~ 7% of the between-participant variability in 6-min walk gait speed and chair rise time, respectively (p < 0.02) (Fig. 1). Lean mass uniquely explained 32% and ~ 4% of the between-participant variance in stair climb power and the time to complete the complex functional task (p < 0.02) (Fig. 1). In addition, the VIF values for each predictor variable are acceptable with all values under 2.2.
Men only model results (Table 3)
The results when only men were included in the models are displayed in Table 3. The overall R2 values explained by the four square step test, muscle strength, and lean mass, for 6-min walk gait speed, stair climb power, 5x chair rise time, and the time to complete the complex function task, were 0.47, 0.55, 0.52, and 0.29, respectively (p < 0.05). Lean mass uniquely explained 23–31% of the between-participant variance for 6-min walk gait speed, stair climb power, and the time to complete the complex function task (p < 0.01). Muscle strength uniquely explained 40% of the between-participant variance for the chair rise time (p < 0.01). The four square step test uniquely explained ~ 12% of the between-participant variance for stair climb power (p < 0.01). In addition, the VIF values for each predictor variable are acceptable with all values under 1.6.
Women only model results (Table 4)
The results when only women were included in the models are displayed in Table 4. The overall R2 values explained by the four square step test, muscle strength, and lean mass, for 6-min walk gait speed, stair climb power, 5x chair rise time, and the complex function task test, were 0.76, 0.42, 0.55, and 0.80, respectively (p < 0.01). The four square step test uniquely explained between 11 and 29% of the between-participant variance for all the mobility tasks (p < 0.01). Muscle strength uniquely explained 4–17% of the between-participant variance for 6-min walk gait speed, 5x chair rise time, and the complex function task test, respectively (p < 0.01). Lean mass uniquely explained 15% of the between-participant variance for the time to complete the complex functional test (p < 0.01). In addition, the VIF values for each predictor variable are acceptable with all values under 2.2.
In this study, we examined the relative contribution of the constructs of muscle strength and lean mass, when compared to the four square step test (as index of lower extremity motor function), in explaining between-participant variance in measures of mobility in older adults. Our a priori hypothesis was the four square step test would be an independent predictor of mobility performance in community-dwelling older adults. Overall, our results are consistent with this hypothesis and underscore the role of lower extremity motor function as a factor in mobility among older adults. Specifically, when data were aggregated by sex, the four square step test was the strongest predictor variable for all the functional tasks, except stair climb power, where the predictor variable representing lean mass (i.e., appendicular lean mass) had the highest contribution. Interestingly, when data were disaggregated by sex, we found that for men, the predictor variables representing lean mass were the strongest contributors, except for 5x chair rise time, where the predictor variable representing muscle strength (i.e., isometric strength by body weight) had the highest contribution. We should note upfront that we were underpowered for sex-specific comparisons, as we had 89 participants with only 29 males, and our predictor variables contained a fair amount of shared variance in some models. As such, our sex-specific findings should be interpreted with caution. In addition, a low spr2 in a given variable should not be interpreted to suggest that it was not an important determinant of mobility, per se, but rather that it did not uniquely explain the between-participant variance. Below we further discuss these findings.
As noted in the introduction, prior research on mobility limitation in older adults has focused largely on factors related to musculoskeletal mechanisms and processes, namely the role of lean mass and muscle strength [35,36,37,38,39,40]. Indeed, numerous studies demonstrate links between the amount of lean mass, muscle strength, and mobility/physical function. For instance, Janssen et al. (2002)  sought to test the hypothesis that low lean mass is related to functional impairment and physical disability in older adults. These authors reported an increased likelihood of functional impairment in older men and women with a reduction in lean mass of 31 and 22%, respectively. Visser and colleagues (2005)  investigated the independent and joint contributions of low lean mass, low muscle strength, and greater fat infiltration into the muscle on incident mobility limitations in older adults. These authors found an association between low lean mass (smaller cross-sectional muscle area) and functional decline, which was a function of underlying muscle strength. Similarly, Reid et al. (2008)  reported lower extremity lean mass to be an important determinant of physical performance among functionally-limited elderly adults. In addition, Curcio et al. (2016)  investigated the relationship between lean mass, muscle strength, and a composite measure of mobility (Tinetti Mobility Test) in non-institutionalized older adults. These authors found a significant linear relationship between lean mass (r = 0.61; measured via bioelectrical impedance analysis [BIA]) and scores on the Tinetti mobility test, as well as between relative strength (r = 0.53; determined via grip strength by BMI) and scores on the Tinetti mobility test. However, recent meta-analyses and studies pooling data across several large epidemiological studies indicate the contribution of muscle mass to mobility appears to be considerably less than originally postulated [13, 39]. For instance, Schapp et al. (2013) , conducted a meta-analysis of 50 studies and noted that lower muscle strength, as well as a high BMI (> 30), were associated with functional decline, but low lean mass was not significantly associated with functional decline. Most recently, the Sarcopenia Definitions and Outcomes Consortium group, which assembled data from eight Epidemiological Cohorts (n = 18,831), reported that grip strength (expressed in absolute terms and relative to BMI) was a discriminator of risk for mobility disability (walking speed < 0.8 m/s), but that DEXA-derived lean mass measures were not good discriminators of mobility disability . Our findings extend this prior work and suggest the contribution of lean mass and muscle strength vary depending on the task, in addition to likelihood of varying by sex.
We also interpret our findings to suggest that one reason the current sarcopenia variables of mass and strength do not adequately discriminate, or predict, mobility limitations as they are insufficient to explain “motor function”. Safe and effective negotiation of complex environments requires the integration of sensory information with neural networks, which involves cortical, subcortical, brainstem, and spinal cord structures . Considering the multifactorial nature of mobility, numerous neural substrates and circuits are implicated in age-related mobility declines . We contend that the reason the four square step test is a unique determinant of mobility capacity in our study is because it heavily challenges motor planning and initiation, as well as motor sequencing and recall, and incorporates musculoskeletal, and likely, peripheral sensory factors that standard knee extensor strength and mass tests do not evaluate. Choice stepping reaction time tests, such as the four square step test, have been shown to discriminate older adults who have fallen, from those who have not [15, 43, 44]. Within this context, we should further note that the four square step test alone demonstrated an area under the receiver operating curve of 0.83 for discriminating those whose gait speed was ≤0.80 m/sec during the 6 min walk test, in comparison to those whose gait speed was > 0.80 m/sec (data not shown in the results due to the small sample size).
The four square step test has been used in the study of a number of neurological diseases (e.g., Parkinson’s disease, multiple sclerosis, and cerebral palsy), as it demands complex anticipatory postural adjustments for gait initiation, known to be impaired with these populations [45,46,47]. However, it has not been widely used in the fields of aging systems and geriatrics. Considering that the test is clinically viable (i.e., it is quick [2–3 min], cheap, and easy to perform), we encourage future investigations, particularly the large cohort epidemiological studies, to incorporate this measure to examine whether it increases the sensitivity and specificity of the current sarcopenia variables of mass and strength in discriminating and predicting mobility limitations in older adults.
As previously mentioned, the relative contribution of the various predictor variables differed depending on the task in question. We purposely chose tasks that measured different aspects of mobility, including locomotor and non-locomotor tasks. As such, it is not surprising that the relative contribution varied by task. Again, we caution about interpretation as this should not be taken to suggest that a given variable is not an important determinant for measure of mobility, per se, but rather that it did not uniquely explain the between-participant variance. We should also note the relative contribution of the predictors varied by sex and task. This raises the question of: Do the determinants for measures of mobility limitations differ between sex? Our sample size is too small to definitively answer this question, but our findings certainly suggest that future work along this line of questioning is warranted.
There are limitations of this work that should be acknowledged. First, there could be better methods and/or approaches to assay motor function, strength and/or mass, as well as assessing measures of mobility. To counter this limitation, we expressed our lean mass and muscle strength data in a variety of ways to maximize their opportunity for these constructs to contribute to the respective models. Within this context we should also note that we, and others, have recently questioned the use of DEXA for assessing muscle mass over time [24, 48, 49]. However, DEXA is tightly correlated with gold standard measures of mass via magnetic resonance imaging (MRI) and computerized tomography (CT) scans cross-sectionally [24, 50,51,52]. Second, our sample size is relatively small for a study of this nature. Moreover, our sample size of men was roughly half that of women. Thus, our sex-specific results are underpowered and should be interpreted with caution. Lastly, we should note that this was a cross-sectional study design, which has obvious limitations in comparison to longitudinal studies.
We sought to determine the relative contribution for indices of muscle strength and lean mass, and lower extremity motor function in explaining between-participant variance in measures of mobility in older adults. Our a priori hypothesis that the four square step test would uniquely explain the between-participant variance in measures of mobility above and beyond that observed with muscle strength and mass was supported. These findings underscore the multifactorial role of lower extremity motor function as an important factor in mobility function in older adults, though the degree of unexplained variance indicates that further work is needed to identify new factors.
Availability of data and materials
All data generated or analyzed during this study are available from the corresponding author on reasonable request.
Akaike’s Information Criterion
Bioelectrical Impedance Analysis
Body Mass Index
Complex Functional Task
Magnetic Resonance Imaging
Mallows’ Prediction Criterion
Maximum Voluntary Contraction
Repeatable Battery for the Assessment of Neuropsychological Status
Schwarz Bayesian Criterion
Short Physical Performance Battery
Statistical Package for Social Sciences
Variance Inflation Factor
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This work was supported in part by the National Institute on Aging at National Institute of Health (NIH), under award number R01 AG044424 (to BC Clark). The content is solely the responsibility of the authors and does not represent the official views of the NIH. The sponsor had no role in the design and conduct of the study; collection, management, analysis, and interpretation of the data; preparation, review, or approval of the manuscript; and decision to submit the manuscript for publication.
Ethics approval and consent to participate
The Ohio University Institutional Review Board approved this study, and all study participants had to provide written informed consent in accordance with the Declaration of Helsinki for their participation. Furthermore, this study adheres to the CONSORT guidelines.
Consent for publication
In the past 5-years, Brian Clark has received research funding from the NIH, Regeneron Pharmaceuticals, Astellas Pharma Global Development, Inc., RTI Health Solutions, Biophytis, and the Osteopathic Heritage Foundations. In the past 5-years, Brian Clark has received consulting fees from Regeneron Pharmaceuticals, Abbott Laboratories, and the Gerson Lehrman Group. Additionally, Brian Clark is co-founder with equity, and serves as the Chief of Aging Research, of AEIOU Scientific, LLC. In the past 5-years, Todd Manini has received research funding from the NIH, Regeneron Pharmaceuticals and Sanofi Pharmaceuticals for contracted studies that involved muscle related research. Todd Manini also owns publicly traded stock in Abbott Laboratories and Amgen Inc. who both make muscle health related products. Purchase of these stocks occurred prior to the beginning of the current research. In the past five years, David Russ has received research funding and consulting fees from Abbott Nutrition. All other authors declare no conflicts of interest, financial or otherwise.
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Wages, N.P., Simon, J.E., Clark, L.A. et al. Relative contribution of muscle strength, lean mass, and lower extremity motor function in explaining between-person variance in mobility in older adults. BMC Geriatr 20, 255 (2020). https://doi.org/10.1186/s12877-020-01656-y
- Functional independence
- Physical function