Fig. 4

PTEN inhibitor antagonized the destructive effect of the miR-132 inhibitor on PTEN/PI3K/AKT signaling pathway, cell proliferation, and inflammation divisors of chondrocytes. a Representative western blot bands of PTEN, PI3K, p-Akt, and GAPDH in control, miR-132 inhibitor and miR-132 inhibitor +VO-Ohpic trihydrate group. b Quantitative densitometry of the expression of PTEN protein. c Quantitative densitometry of the expression of PI3K protein. d Quantitative densitometry of the expression of the p-AKT protein. e Cell proliferation of chondrocytes. f Apoptosis of chondrocytes. g Representative western blot bands of Bax, cleaved Caspase-9, cleaved Caspase-3, and GAPDH. h Quantitative densitometry of the expression of Bax protein. i Quantitative densitometry of the expression of Caspase-9 protein. j Quantitative densitometry of the expression of Caspase-3 protein. k IL-1β levels. l IL-6 levels. m IL-18 levels. VO-Ohpic trihydrate is the PTEN inhibitor. **P < 0.01 vs inhibitor NC group; #P < 0.05, ##P < 0.01 vs miR-132 inhibitor group