Table 1 Schedule of enrolment, assessments and close-out

TIMEPOINT

-t₁

t₁

t₂

t₃

ENROLMENT:

Eligibility screen

Informed consent

X

X

ASSESSMENTS:

Functionality

Technological - Activities of Daily Living Questionnaire (T-ADLQ) [30].

X

X

X

Everyday Cognition Scale (ECog) [31].

X

X

X

Pfeffer Functional Activities Questionnaire (PQAF) [29].

X

X

X

Socio-demographic ^a [67]

Marital status.

X

X

X

Education.

X

Occupational background.

X

Ethnicity.

X

Individual and household income.

X

X

X

Assets inventory.

X

X

X

Health insurance.

X

X

X

Household conformation.

X

X

X

Social network information.

X

X

X

Health, risk factors, anthropometric and laboratory assessment ^b

Health related quality of life (EQ-5D) [32].

X

X

X

Tabacum and alcohol consumption (Alcohol Use Disorders Identification Test, AUDIT) [33].

X

X

X

Audition and vision section of the Chilean National Health Survey [34].

X

X

X

Physical activity, sedentarism and diet [34].

X

X

X

Oral health thought the Oral Health Impact Profile (OHIP).

X

X

X

Frailty: Fried Frailty Phenotype and the Frail Questionnaires [35, 36].

X

X

X

Anthropometric measurements: weight, body mass index (BMI), systolic and diastolic blood pressure (seat and standing).

X

X

X

Framingham Cardiovascular Risk Scale.

X

X

X

Laboratory evaluation: hemogram, glycaemia, lipid profile, level of vitamin B12 and folic acid, thyroid hormone (TSH and free T4) and hepatic profile.

X

X

X

Health inventory on 18 health conditions (including cardiovascular events).

X

X

X

Psychological assessment ^c

Engagement in stimulating activities.

X

X

X

Ageing related losses.

X

X

X

Personality traits [37, 38].

X

X

X

Psychological well-being [39].

X

X

X

Geriatric Depression Scale - Brink and Yesavage [40].

X

X

X

Depression, Anxiety and Stress Scale (DASS-21) [41].

X

X

X

Coping processes [42].

X

X

X

Social integration.

X

X

X

Cognitive reserve scale [43].

X

X

X

Stage and clinical symptoms ^d

Clinical Dementia Rating for Frontotemporal Lobar Degeneration (CDR-FTLD)-eight domains [44].

X

X

X

Alzheimer Disease- 8 (AD8) [45, 46].

X

X

X

Neuropsychological evaluation

Global Cognitive Function:

- Minimental-State Examination (MMSE) [47].

X

X

X

- Montreal Cognitive Examination (MoCA) [48].

X

X

X

- Addenbrooke’s Cognitive Examination (ACE III) [49].

X

X

X

Memory:

- Short Term Memory Binding Test [50, 51].

- Free and Cued Selective Reminding Test (FCRST) [52,53,54]

- Supermarket task [55].

X

X

X

Executive functions:

- Ineco Frontal Screening [56].

- Verbal fluency test [57].

- Color Trail Test Part B [58, 59].

X

X

X

Language: Sydney Language Battery (Sydbat) [60].

X

X

X

Visuo-constructive abilities: Rey Complex Fig [61, 62]..

X

X

X

Social Cognition: MiniSea [63].

X

X

X

Motor assessment ^e

Soft neurological signs:

-Heidelberg Neurological Soft Signs [64].

-Edinburgh Motor Assessment (EMAS) [65].

X

X

X

Balance: simple-task, dual-task (including cognitive task), and sensorimotor task.

X

X

X

Walking assessment: carrying a cup with water, and counting backwards from 100.

X

X

X

Other scales:

- Tinetti test [66].

- Activities-Specific Balance Confidence Scale (ABC) [67].

- Timed up and go [68].

X

X

X

Neuroimaging ^f

Three whole-brain sequences:

- High-resolution T1-weighted magnetic resonance image (MRI).

- Resting-state functional magnetic resonance images (RS-fMRI)

- Diffusion tensor-based images (DTI).

- Axial T2 and Flair sequences to detect infarcts and white-matter alterations.

X

X

Gut microbiome

16S analysis from stool samples [69].

X

X

X

Biomarkers

Six inflammatory biomarkers, IL-2, IL-6, IL-10, TNFα, SAP and CRP [70,71,72,73,74].

X

X

Genetic study

Family pedigree through a questionnaire in accordance to Goldman criteria [75].

X

Candidate genes associated with neurodegenerative diseases (ApoE, TREM2 and MAPT) using real time PCR analysis.

X

Genome-Wide Association Study (GWAS) [76].

X