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Fig. 1 | BMC Geriatrics

Fig. 1

From: miR-96, miR-145 and miR-9 expression increases, and IGF-1R and FOXO1 expression decreases in peripheral blood mononuclear cells of aging humans

Fig. 1

miR-96 and miR-182 interact with the 3’UTR of IGF-1R mRNA. HEK 293 cells were co-transfected with the pmirGLO reporter vector and miRNA precursors. The mean relative luminescence induced by firefly luciferase expressed from the reporter vectors containing cloned DNA corresponding to the 3’UTR fragments, in the presence of negative control miRNA, was normalized to 100%. miR-96 interacts with two (a, b), and miR-182 with one out of two in silico indicated binding sites (c, d). pmirGLO: “empty” reporter vector; pmirGLO_IGF-1R_5’: reporter vector containing DNA corresponding to the 5’ end of 3’UTR of IGF-1R mRNA; pmirGLO_IGF-1R_3’: reporter vector containing DNA corresponding to the 3’ end of 3’UTR of IGF-1R mRNA; pre-miR-neg, pre-miR-96, pre-miR-182: miRNA precursors

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BMC Geriatrics

ISSN: 1471-2318

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