Table 6 Secondary outcome measures continued: Health status

Health Status

Habitual Physical Activity level

Daily physical activity, sedentary behaviour and sleep quality and quantity are measured with two Actigraph monitors worn for seven days. The actigraph on the waist measures physical activity and the wrist sleep quality, with data analysed using the ActilifeGTIM (version 2.2.3) and ActiWebClient (version 4.2.2) programs.

Cortisol

Five saliva cortisol samples will be collected using the Salivette (Sarstedt Aktiengessllschaft and Company; Kirschbaum & Hellhammer, 1994) and according to manufacturer instructions. A control 'resting' "Pre" and "Post" samples will be taken before exposure to each 'stressor' condition fasting and upon awakening and 30 minute rest, and a prior to stressor and immediately after the termination of the stressor condition. The cognitive 'stressor' condition is Alzheimer's Disease Assessment Scale (ADAS-Cog) assessment during screening and at 6 months assessment (approximately 45 min), the physical stressor is the second exposure to a set of maximal strength testing (1RM testing) at baseline and 6 mo.

Medical History

Physician complete medical history and physical and neurological examination.

Inflammatory/Anabolic/Deoxyribo-Nucleic Acid (DNA) profile

Serum samples for nutritional, biochemical and hormonal factors, pro- and anti-inflammatory cytokines

A venous blood draw is taken after a 12 hour fast for B12, folate, Thyroid Stimulating Hormone (TSH), insulin, glucose (with calculation of insulin sensitivity and beta−cell function using the HOMA2 Computer Model), Liver Function Tests (LFT), cholesterol (Total, High Density Lipoprotein, Low Density Lipoprotein, Triglycerides), Full blood count, creatinine, albumin, homocysteine, and 25−OH vitamin D level, and a second sample taken to measure specialist markers of inflammation: high sensitivity C−reactive protein (hs−CRP), cytokines InterLeukin (IL)−1b, IL−6, IL−8, IL−10, IL−12p70, IL−18 and Tumor Necrosis Factor− proteins (TNF-proteins), as well as Brain Derived Neurotrophic Factor (BDNF), Insulin Growth Factor-1 (IGF-1). At Baseline only a blood draw is taken for genetic Testing for Apolipoprotein allele 4 (APOE 4).