Fig. 3

Potential mechanisms of the asYb8c4^ins-mediated suppression of ALDH1A1 expression. A Distribution schematic of the CpG island and sites in intron 4 of ALDH1A1. B Methylation levels of the asYb8c4^ins-introduced CpG island in peripheral blood of PD cases and controls (n = 5). C-D Methylation levels of upstream (C) and downstream (D) CpG sites in peripheral blood of subjects carrying the asYb8c4^ins/ins or asYb8c4^del/del genotype (n = 5). E Reverse-transcribed PCR products amplified between ALDH1A1 exons 3 and 5 from peripheral blood of subjects carrying the asYb8c4^ins/ins or asYb8c4^del/del genotype. F Silver staining gel of the DNA pull-down products from nuclear extract of SK-N-SH cells. Arrows indicate the protein bands used for mass-spectrometry identification. asYb8c4^del antisense Yb8c4 deletion, asYb8c4^ins antisense Yb8c4 insertion, PD Parkinson’s disease