Fig. 2

Impact of asYb8c4^ins and asYb8c4^del on gene expression. A mRNA expression of ALDH1A1 in PBMCs of different genotype carriers (n = 14 for asYb8c4^del/del; n = 15 for asYb8c4^ins/ins). Results were normalized to their respective ACTB levels. B Schematic diagram of the luciferase reporter and EGFP constructs containing asYb8c4^ins (-123 to + 406), asYb8c4^del (-123 to + 106), and a long fragment with asYb8c4^del (L-asYb8c4^del; -274 to + 262; length similar to asYb8c4^ins). The position + 1 denotes the base immediately before asYb8c4^ins. The indicated fragments were amplified from intron 4 of ALDH1A1. C-D Effect of asYb8c4^ins, asYb8c4^del and L-asYb8c4^del on the firefly luciferase activity (C) and mRNA levels (D). Results were normalized to their respective Renilla luciferase activity or mRNA levels (n = 3). E Effect of asYb8c4^ins and asYb8c4^del on EGFP expression (n = 3). *, P < 0.05. asYb8c4^del antisense Yb8c4 deletion, asYb8c4^ins antisense Yb8c4 insertion, PBMC peripheral blood mononuclear cell