Fig. 1

ABT-263 treatment improved the survival rate of sepsis in the aged mouse which may relate to the regulation of autophagy. a animal experiment design for the survival curve of sepsis. b-c The survival rates of the aged mouse which induced sepsis by cecal slurry injection (CSI) after treated by ABT-263, Vehicle and 3-MA, respectively (14 per group). The survival rate of ABT group was 71.4% (10/14), while the control group (CSI) was 42.9% (6/14), the 3-MA group was 50% (7/14). The difference between ABT and CSI group was significant (P = 0.038). c The survival rates of the young mouse induced sepsis which received the same treatments as the aged mouse. The survival rates of ABT, CSI and 3-MA were 71.4% (10/14), 57.1% (8/14) and 64.3% (9/14), respectively. How, no significant difference between those 3 groups. d-e SA- β -gal staining assay was performed in peritoneal macrophages from ABT-263 treated and Aged groups. The blue stained cells (white arrow) indicated the senescent cells and the proportions were presented in the panel e. ** indicated P < 0.05 between the two groups. f RNA isolated from the mouse spleen from group ABT and CSI was characterized on the Mouse RT2 Profiler PCR Array. The volcano plot of the PCR Array identified significant gene expression changes. The plot displayed statistical differences (P < 0.05) versus fold-change (Group ABT vs. CSI) on the y and x-axes, respectively. g Gene expression changes in group ABT vs. CSI. Data was plotted as relative up-regulation or down-regulation on ABT vs. CSI (P < 0.05). Each column is one gene and represents data from three samples for each genotype. The full gene names, their functions and p-value please refer to supplementary Table 1