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Figure 1 | BMC Geriatrics

Figure 1

From: Identifying sexual differentiation genes that affect Drosophila life span

Figure 1

Drosophila sex determination hierarchy and the tudor mutation. A) The Drosophila somatic sex determination hierarchy. The X to autosome ratio causes Sxl to be "off" in males (indicated in gray color) and to be "on" in females. In males, lack of SXL and TRA activity leads to expression of FRUM and DSXM . In females, TRA and TRA2 together direct splicing of downstream targets to produce DSXF , and fru P1 transcripts that are not translated in females. B) Crossing scheme to produce germ line-ablated flies using tudor[1]. For the experimental group, tudor homozygote females were crossed to wild type Oregon-R males to generate progeny lacking a germ line. For the control group, tudor heterozygote females were crossed to Oregon-R males to generate progeny containing a normal germ line. Both experimental and control groups have the same chromosomal composition. B) Test for Wolbachia. The Wolbachia 16S rDNA sequences were amplified by PCR from the indicated Drosophila lines and controls, and the presence or absence of Wolbachia-specific PCR products was determined by gel electrophoresis and ethidium bromide staining.

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