Table 1 Main characteristics of the analytical methods used for the laboratory tests
Test (unit) | Analyser/kits | Matrix | Reference range | Reproducibility CV% | LOD |
|---|---|---|---|---|---|
BNP (pg/mL) | DxI 800/Biosite | Plasma EDTA | 5-100 | 5.4-6.7 | 1 |
NT-proBNP (pg/mL) | Dimension/Siemens | Serum | < 450 (> 75 yrs) | 3.9-4.3 | NR |
TSH (μU/mL) | DxI 800/Beckman | Serum | 0.2-3.5 | 3.72-4.96 | 0.003 |
Free T4 (ng/dL) | DxI 800/Beckman | Serum | 0.9-1.8 | 4.3-8.8 | 0.5 |
Parathyroid hormone (pg/ml) | DxI 800/Beckman | Serum | 16-81 | 2.8-6.4 | 1 |
Creatinine (mg/dL) | DxC 800/Beckman | Serum | 0.6-1.4 | 1.7-9.5 | 0.05 |
Cystatin C (mg/L) | DxC 800/Gentian | Serum | 0.5-1.0 | 2.95 - 4.38 | <0.1 |
HDL (mg/dL) | DxC 800/Beckman | Plasma heparin | > 60 | 2.1-3.4 | 5 |
Total cholesterol (mg/dL) | DxC 800/Beckman | Plasma heparin | < 200 | 1.48-1.6 | 5 |
Triglycerides (mg/dL) | DxC 800/Beckman | Plasma heparin | < 150 | 2.2-2.6 | 10 |
Haemoglobin (g/dL) | Sysmex XE-2100 | Whole blood | 12-17 | 0.5-1.0 | <1.0 |
Calcium (mg/dL) | DxC 800/Beckman | Serum | 8.6-10.0 | 1.0-2.2 | 2 |
Phosphorus (mg/dL) | DxC 800/Beckman | Serum | 2.4-4.7 | 0.6-3.0 | 0.5 |
Transthyretin (mg/dL) | DxC 800/Beckman | Serum | 18-38 | 3.6-4.2 | 2 |
usCRP (mg/dL) | DxC 800/Beckman | Serum | < 0.3 | 4.1-5.1 | 0.02 |
CMV IgG (A.U./L) | Architect i4000/Abbott | Plasma EDTA | < 6 (normal) 6-15 (suspicion) | 5.9-6.2 | NA |
25-OH-Vitamin D (ng/mL) | Liaison/Diasorin | Serum | 30-100 | 5.2-8.4 | < 5.0 |
IGF1 (ng/mL) | Liaison/Diasorin | Serum | 70-140 (> 75 yrs) | 5.8-6.1 | < 15 |
Osteocalcin (ng/mL) | Liaison/Diasorin | Serum | 3-7 | 7.4-7.6 | < 1.0 |
Aluminium (μg/dL) | ICP-MS, Agilent/NA | Plasma heparin* | 0.2-1.0 | 8.9 | 0.1 |
Copper (μg/dL) | ICP-MS, Agilent/NA | Plasma heparin* | 70-170 | 1.22 | 3.0 |
Zinc (μg/dL) | ICP-MS, Agilent/NA | Plasma heparin* | 70-120 | 1.39 | 5 |
Selenium (μg/dL) | ICP-MS, Agilent/NA | Plasma heparin* | 5-15 | 1.18 | 0.5 |
CC16 | ELISA | Serum | 2-20 | 4-9 | 1.0 |
SPD | ELISA | Serum | 50-150 | 4.2-9.8 | 0.2 |